Gluten Free Wine & Dine Chicken $13.00 Roasted chicken breast, exotic mushrooms, drizzled with red wine balsamic reduction, served with red pepper mashed potatoes and Chef?s vegetables. Gluten Free Slow Smoked BBQ Ribs – Full $19.00 Glazed in your choice of mn nice BBQ, hellcat inferno or black cat diablo, served with red pepper mashed potatoes, and pickled slaw. Gluten Free Entrees Fish Tacos $12.00 Three hard shell tacos stuffed with white fish, sambal sauce, lime, dry aged cheese, pico de gallo & roasted corn, served with a mixed greens salad Slow Smoked BBQ Ribs – Half $14.00 Glazed in your choice of mn nice BBQ, hellcat inferno or black cat diablo, served with red pepper mashed potatoes, and pickled slaw. Gluten Free Local Beet Salad $11.00 Roasted local beets, mixed greens, red onions, goat cheese, candied macadamia nuts, rosemary vinaigrette. Gluten Free Hellcat Inferno Salad $13.00 Inferno sauce glazed chicken breast, tostada with cumin black beans layered on top of mixed greens, red & green bell peppers, red onions, toasted pumpkin seeds, drizzled with fajita ranch dressing, r Chop Chop Salad $12.00 Romaine lettuce, grilled chicken, crisped bacon, smoked bleu cheese, crisp bell peppers, red onions, tomato, hard boiled eggs & garlic croutons, and your choice of dressings. Gluten Free Cinco de Mayo Dip $10.00 Cumin black bean spread, cheddar, fresh avocado, roasted red pepper sour cream, pickled jalapenos, pico de gallo, crispy tortilla chips Pub Fries $6.00 Crispy seasoned fries with a big potato flavor on the inside and finished with Chatterbox spice blend Soup & Salad Dressings are homemade signature recipes: Bleu cheese & scallion, fajita ranch, honey french, rosemary vinaigrette Home Made Chili $7.00 Chef?s Homemade Soup $6.00 Bowl of Soup & Side Salad $9.00 Side Salad $6.00 Mixed greens, parmesan, tomatoes, bell peppers, onions, garlic croutons, and your choice of dressings. Served with a salt crusted warm pretzel and fresh vegetables for dipping Smokin Wings $10.00 House smoked, deep fried wings, served with your choice of mn nice BBQ, hellcat inferno, black cat diablo or house buffalo. Related Restaurants 2022: Starters Chicken Quesadilla $10.00 Flour tortilla stuffed with roasted chicken, cilantro-pumpkinseed pesto, onions, peppers, cheddar, roasted red pepper sour cream & pico de gallo Spinach & Artichoke Dip $9.00 Roasted garlic, parmesan, ricotta, feta, topped with tomatoes, hand made toasted bread Pretzel & Beer Cheese Fondue $10.00 Creamy blend of cheddar cheese, smoked onions, spices and Chatterbox Speakeasy Lager.
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Riven used to be one of my favorite games.
In that sector, the company tops Microsoft, which controls 18%, and Google, which claims 9%. In recent years, the AWS division has partnered with Pinterest, Slack and Lift.Īs of mid-2020, Amazon controls 33% of the world's cloud infrastructure services market. He has conducted business with the Central Intelligence Agency and the Democratic National Committee. Insider spoke with over 10 current and former employees to get their initial reactions. Jassy’s success has stemmed from his ability to attract consumers to Amazon’s cloud computing products. Amazon Web Services CEO Andy Jassy will replace Jeff Bezos as Amazon CEO later this year. He currently owns 85,000 shares of Amazon valued at $287.3 million. Jassy, who succeeds Jeff Bezos as Amazon’s chief executive officer on July 5, is steeped in the company’s corporate religion: Put customers first, move fast, be frugal. In 2018, he earned $19.7 million, most of which was from stock awards. In 2019, Jassy earned $348,809 in compensation from Amazon. The 17 other S-team members include finance chief Brian Olsavsky and Charlie Bill, senior vice president of Amazon's Utility Computing Services. The 53-year-old Jassy is a member of Amazon's S-team, which is Amazon's elite group of executives. The AWS division continues to drive much of Amazon’s profit. His work with AWS helped the e-commerce company grow into a technology giant. Jassy joined the company in '97, and has led Amazon’s Web Services cloud team for the past 15 years. His new role makes him the second CEO in Amazon’s 27-year history. Jassy graduated from Harvard University in 1990 and Harvard Business School seven years later. Jassy, who succeeds Jeff Bezos as Amazon’s chief executive on July 5, is steeped in the company’s corporate religion: Put customers first, move fast, be frugal. He will be an outstanding leader, and he has my full confidence.” Andy is well known inside the company and has been at Amazon almost as long as I have. Jeff Bezos to step down as Amazon chief executive. “In the Exec Chair role, I intend to focus my energies and attention on new products and early initiatives. However, Andy Jassy, 53, is reported to be a close confidant of Mr Bezos. “I’m excited to announce that this Q3 I’ll transition to Executive Chair of the Amazon Board and Andy Jassy will become CEO,” Bezos wrote in a statement. His replacement, Jassy, is the company’s top cloud executive. He is a member of Bezos' elite S-team of executivesĪmazon is set to welcome a new chief executive officer, Andy Jassy, following Jeff Bezos' announcement on Tuesday that he is stepping down as CEO in the third quarter.īezos, 57, will exit his post with the e-commerce colussus in order to transition to executive chairman of Amazon’s board.Jassy has led Amazon's Web Services division since 1997.Bezos will turn the helm over to Andy Jassy.
Screens on screened-in porch replaced and reframed in 2020. Water radon filter/mitigation system upgraded in 2020. Back yard was leveled and irrigated by adding an underground drainage system to create useable land, providing an additional 500 sq. The Zestimate for this house is 575,100, which has increased by 12,323 in the last 30 days. The full finished lower level adds another 415 sq/ft of living space. 27 Clearview Dr, Sandy Hook, CT is a single family home that contains 2,181 sq ft and was built in 1982. Master bedroom features walk-in closet and private bathroom. Upstairs, meet a newly-updated full bath with whitewashed herringbone porcelain wood look tile flooring and Carrara marble subway wall/ceiling tile in the shower/tub combo. Stairs to the second level feature a carpet runner which marries the stairs to the upstairs hallway and bedrooms. Living room features an additional wood burning fireplace. Stunning brick fireplace greets visitors in the sitting room, opposite a bright, spacious bay window offering a clear view of the backyard. The main floor features beautiful living space with gleaming hardwood floors replaced in 2014. Wall separating the dining room from the sitting area removed to create an expansive, open layout perfect for family gatherings and socializing. The gourmet eat-in kitchen includes granite counters, a built-in desk area, and stunning authentic wood beams spanning the length of the ceiling. Both rooms have their own set of french doors.Here's your opportunity to call one of Newtown's most desirable neighborhoods home. Uh-oh Este propiedad no se encuentra actualmente en alquiler. 27 Clearview Dr 27 Clearview Dr Newtown, CT 06482. The wetbar is a well designed feature that is functional in the Dining/Living Rm area and opens to both the beautiful kitchen & vaulted family Rm. 27 Clearview Dr y otros apartamentos y casas cercanas en Newtown, CT Vea rentas oficiales, fotos, planos de piso y características de apartamentos, casas y condos en alquiler cerca de 27 Clearview Dr. This room can be designed to showcase an incredible farmhouse dining table designed for large family gatherings along side the warmth & glow of a blazing fire or separate the room in two with a more intimate dining area & informal living area. The piece d'resistance are the stunning built-in illuminated cabinetry with rich wood countertops, glass cabinet doors that can serve as the perfect buffet, china credenza-you decide. Nicely updated 4 bedroom, 2.5 bathroom Colonial with a 14 x 28. The main dining room/living room with its fireplace is the heart of the home with vaulted ceilings/overhead balcony. house located at 22 Clearview Dr, Newtown, CT 06482 sold for 399,900 on Jun 27, 2014. Almost every room features french doors that allow you to enjoy your surroundings & outdoors. The main level offers a fabulous open flr plan perfect for entertaining. Take notice of the dog house w Air Conditioning! Stone steps & stone walkway lead you inside to rich wide planked hrdwd flrs. Both curb appeal and privacy co-exist with a lovely yard for sprawling & recreation. This is your chance to live in a home where the amenities & craftsmanship have been thoughtfully designed. A special home indeed! Rarely will you see these these types of features & built-ins in a home at this price point. In immunogenicity studies among people 18 years and older and 4 through 17 years old, Flucelvax Quadrivalent was found to produce a similar immune response to the trivalent formulation. What were the results of the clinical trials using cell-based technology?Ī clinical trial of the previous trivalent formulation of Flucelvax demonstrated effectiveness and safety among persons 18 through 49 years old. Cell-based vaccines might be easier to scale up if egg supply is limited. Growing CVVs in cell culture for the manufacture of Flucelvax Quadrivalent is not dependent on an egg supply. The cells used to manufacture Flucelvax Quadrivalent are kept frozen and “banked.” Cell banking ensures an adequate supply of cells is readily available for vaccine production. Some observational studies have shown greater protection against flu or flu-like illness among people who received cell-based inactivated influenza vaccines compared with those who received standard-dose egg-based inactivated vaccines.Ī potential additional advantage of cell-based technology is that it might permit faster start-up of the vaccine manufacturing process in the event of a pandemic. What are the possible benefits of using cell-based flu vaccines? FDA’s approval of cell-based CVVs for use in cell-based flu vaccines could possibly improve the effectiveness of cell-based flu vaccines. For example, egg-adapted changes could result in production of less effective antibodies by the human immune system, and this can make vaccines less effective at preventing disease caused by the specific flu viruses in circulation. These changes may have important implications for the body’s immune response to vaccination. Growing flu viruses in eggs can introduce changes (called egg-adapted changes) that can cause differences between the viruses in the vaccine and the ones that are circulating (‘wild’). What is the significance of FDA approving cell-based candidate vaccine viruses for use in the Flucelvax Quadrivalent cell-based flu vaccines? Learn more about the cell-based flu vaccine manufacturing process on CDC’s How Flu Vaccines are Made web page. Since the 2019-2020 flu season, all four of the CVVs provided to the cell-based vaccine manufacturer are cell-derived rather than egg-derived. The cell-based vaccine manufacturing process uses mammalian cells (Madin-Darby Canine Kidney, or MDCK cells) to grow flu viruses instead of fertilized hen’s eggs. How is the cell-based vaccine manufacturing process different than the traditional egg-based manufacturing process? Since the 2019-2020 flu season, all four flu CVVs used in the Flucelvax Quadrivalent are cell-based, making the vaccine egg-free. However, this difference was not seen in similar studies conducted during the 20 flu seasons. In one study published in the Journal of Infectious Diseases among Medicare beneficiaries 65 years and older during the 2017-2018 influenza season, cell-based vaccine provided greater protection against flu-related hospitalizations than standard-dose, egg-based vaccine. The viruses used to make cell-based vaccines might be more similar to circulating “wild” flu viruses than the viruses grown in eggs and used to make egg-based vaccines. In addition, use of cell-based candidate vaccine viruses (CVVs) in vaccine production has the potential to offer better protection compared to traditional, egg-based flu vaccines. Why has a cell-based flu vaccine been developed?Ĭell-based flu vaccine production does not use flu viruses grown in eggs and, therefore, is not dependent on the supply of eggs. Some people who have had an allergic reaction to other kinds of flu vaccines (other than cell-culture based flu vaccines vaccines) might be able to get Flucelvax Quadrivalent. People who are not within the FDA-approved age range (those younger than 6 months), those who have had severe allergic reactions to any ingredient of the vaccine, or who have had an allergic reaction to a cell-culture based flu vaccine should not get Flucelvax Quadrivalent. Who should not get Flucelvax Quadrivalent? Who can get Flucelvax Quadrivalent?įlucelvax Quadrivalent is licensed for use in people 6 months and older. Currently, the recombinant flu vaccine and the cell culture-based flu vaccine are the only egg-free flu vaccines licensed for use in the United States. The flu viruses used in the cell-based vaccines are grown in cultured cells of mammalian origin instead of in hen’s eggs.įlucelvax Quadrivalent is the only influenza vaccine in use in the United States for which the vaccine viruses are grown in a mammalian cell culture. Most inactivated flu vaccines are produced by growing flu viruses in eggs. ‘Cell-based’ refers to how the influenza (flu) vaccine is made. Tel: Benefits administers the City’s employee/retiree insurance benefit and pension programs. This office also administers the performance management system and provides performance training and consultation. Tel: and Employee Development is responsible for design, implementation and evaluation of citywide executive, supervisory and employee development programs orientation of new employees literacy and basic skills enhancement team building and the customer service program. Poindexter, Organizational Development Director This office also performs: procurement, financial tasks, Department performance metrics, administrative tasks, and ensures compliance with laws and regulations, governing of open records, authorization to work and unemployment compensation.ĭarrilynn V. Services include: system maintenance of employee records and personnel actions, report production, ERP training, and position management. Tel: Information Systems and Records Management is responsible for the accurate presentation of employee and position data in the human resources information system. Jerese Isaac, HRIS & Master Data Director The office also serves as the point of contact for the city’s labor unions. It also facilitates the city’s civil service system by supervising the filing and scheduling of adverse personnel action appeals filed by regular service, classified employees and all sworn employees in the police and fire departments. To this end, the office conducts internal administrative investigations into allegations of discrimination, harassment, retaliation, workplace violence and bullying. It also supervises the city’s grievance process for classified employees to equitably and timely resolve issues related to supervisory-employee relationships, working conditions, classification and pay, departmental policies and procedures, and discipline. Tel: and Employee Relations provides advice, counsel, and policy direction to all departments to ensure equal treatment and opportunities are extended at every touchpoint of the employee experience. Tamika Spirling-Brooks, Labor Relations/EEOC Director The Talent Acquisition Division also promotes the City’s employment brand across numerous markets and professional communities. Their efforts include sourcing, identifying, pre-screening/qualifying, interviewing, developing offer packages, negotiating and closing candidates. Tel: Acquisition provides recruiting support for all departments across across the City. Contact Informationįor Employee Benefits Related Inquiries, Please Call 40įor Employee Verifications & Student Loan Forgiveness, Please Call 40 The department designs and manages the City’s Human Resources programs and fosters the development of innovative policies and practices. The Department of Human Resources partners with city agencies and employees to hire, compensate, support, and develop a diverse workforce that is dedicated to delivering high-quality services to the community. Ensure compliance with employment-related laws and regulations.Promote fairness and equitable treatment for all applicants and employees.Create a learning environment that sustains a culture of excellence.Promote employee health and financial wellness. The mission of the Department of Human Resources is to attract, retain, and develop a diverse and competent workforce that enables City departments to achieve their business objectives. Commissioner of Human Resources | Tarlesha Williams Smith, Esq. In our test, all samples had fewer than 50% adapter-adapter ligation reads (Additional file 1: Figure S2). PEPPRO counts and reports the fraction of reads that contain adapter-adapter ligation products, then removes adapter sequences and adapter-adapter ligation sequences before downstream alignment. The frequency of adapter-adapter ligation can be reduced by molecular techniques (see the Methods section), but these are not always possible and many experiments retain adapters in high molar excess, leading to substantial adapter-adapter sequences. The second ligation can lead to adapter-adapter ligation products that are amplified by PCR. These methods require two independent ligation steps to fuse distinct RNA adapters to each end of the nascent RNA molecule. Adapter ratioĪ common source of unwanted reads in PRO/GRO/ChRO-seq libraries results from adapter-adapter ligation. Here, we describe each plot and statistic produced by PEPPRO. Results of PEPPRO can be explored in the PEPPRO HTML-based web report, which displays all of the output statistics and QC plots (see PEPPRO documentation). We ran PEPPRO on our public test set, our differential expression test set, and our spike-in set. To demonstrate how PEPPRO responds to mRNA contamination, we also generated a set of 11 samples built from a single PRO-seq library (GSM1480327) that we spiked with increasing amounts of RNA-seq data (GSM765405) (Additional file 1: Figure S1). Thus, PEPPRO provides a unified, cross-platform pipeline for nascent RNA profiling projects. PEPPRO can be easily deployed across multiple samples either locally or via any cluster resource manager, and we also produced a computing environment with all the command-line tools required to run PEPPRO using either docker or singularity with the bulker multi-container environment manager. PEPPRO is compatible with the Portable Encapsulated Projects (PEP) format, which defines a common project metadata description, facilitating interoperability. PEPPRO features include (1) a serial alignment approach to remove ribosomal DNA reads (2) nascent transcription-specific quality control outputs and (3) a modular setup that is easily customizable, allowing modification of individual command settings or even swapping software components by editing human-readable configuration files. Here, we introduce PEPPRO, an analysis pipeline for uniform initial sample processing and novel quality control metrics. While tools are available for downstream analysis, such as to identify novel transcriptional units and bidirectionally transcribed regulatory elements, there is no comprehensive, unified approach to initial sample processing and quality control. With increasing data production, we require analysis pipelines for these data types. These advantages have led to growing adoption of global run-on (GRO-seq), precision run-on (PRO-seq), and, most recently, chromatin run-on (ChRO-seq) experiments. Fourth, nascent RNA profiling can be used to determine pausing and RNA polymerase accumulation within any genomic feature. Third, nascent RNA profiling measures unstable transcripts, which can be used to infer regulatory element activity and identify promoters and enhancers de novo by detecting bidirectional transcription and clustered transcription start sites (TSSs). Second, nascent RNA profiling measures not only RNA polymerase occupancy, but also orientation by default, whereas traditional RNA-seq requires specific library preparation steps to capture orientation. Steady-state transcription levels are commonly measured by RNA-seq, but there are many advantages to quantifying nascent RNA transcripts: First, it measures the transcription process directly, whereas steady-state mRNA levels reflect the balance of mRNA accumulation and turnover. He lived in a basement apartment in his parents' rental house and died in his bed, apparently unaware the world had caught fire, his father said. Eight of the 22 people who died in the fire lived within a mile of the neighborhood, including Michele Way resident Michael Grabow, 40. He said he was aware constantly for the first 90 minutes of having mere seconds left to live.Īll of the roughly 40 homes in the subdivision formed by Michele and Lorraine ways were destroyed in the Tubbs fire. They watched as their world turned to ash and their odds of survival dwindled.īlaine Westfall used his wife's sweater sleeve as a breathing mask, and counted in his head to stave off the panic of getting too little air. John Smihula hunkered down in a metal and concrete shed. Lorraine Way residents Blaine and Rayna Westfall were pinned face-down on the ground by flames on a hillside below their house for three hours. “We were right in the center of the heat of that thing,” said longtime resident Gary Bayless, 69, who managed to escape. We were right in the center of the heat of that thing. The neighborhood on Santa Rosa's northeastern outskirts was one of the first clusters of suburban homes overrun by the Tubbs fire, which began its deadly march in Sonoma County by mowing down ranch homes and rural estates higher up in the canyon of Mark West Creek, in Knights Valley and Mountain Home. The Wilsons were among a handful of residents on a dead-end street in the Michele Way Estates who were stranded in the early hours of the inferno after flames blocked their only exit. 9, severely injured, but alive - their tale of endurance now part of the tattered fabric of a Mark West Springs-area neighborhood striving to recover from the most destructive wildfire in state history. They would emerge in the early hours of Oct. They said their goodbyes, embracing in the frigid water as the Tubbs fire bore down. Trapped at their home north of Santa Rosa by the wildfire that roared across the Mayacamas Mountains last fall, they took refuge in their swimming pool, surviving on gulps of toxic air that singed their lungs between repeated dunks they hoped would ward off burns. By the time they heard the wail of a siren signaling help's arrival in the dark of their fire-stricken neighborhood, Greg and Christina Wilson already had been through the worst. The installers will install CS6 version 13.0 which is unstable and full of bugs. Bridge Output Module, Extendscript toolkit. The are optional downloads for CS6 you can download from Adobe site. They install all that is included in CS6 Photoshop, Bridge and Extension manager, ACR. The perpetual downloads are stand alone Installers. Either Mac or Windows the creative subscription is platform independent and should be installed by the creative cloud desktop application not the stand alone installers. The CS6 serial number and downloads are platform dependant. Your cs6 perpetual serial will activate the version you licensed. The downloads are for the Adobe stand alone Photoshop Installers either the Windows version or the Mac version. (Will this install just the ACR 9.1.1 update or will it also install the DNG Converter 9.1.1?) (It states that this update is highly recommended to be installed through Photoshop, should I just disregard this message?)Īdobe - Bridge : For Windows : Adobe Bridge CS6 5.0.2 Update (64-bit)Īdobe - Adobe Camera Raw and DNG Converter : For Windows : Adobe DNG Converter 9.1.1 Please let me know if these would be the only 3 possible updates for the CS6 installation, if I perform the installation without any extra components.Īdobe - Photoshop : For Windows : Adobe Photoshop 13.0.1.3 update for CS6 I wish to avoid all this by installing the updates manually. I would then have the Creative Cloud as an installed control panel program. This will result in having the Application Manager as a start menu program and if I were to launch it, it would turn into the Creative Cloud. Meaning, if I were to install any updates through Photoshop's help/check for updates, the Application Manager will get installed. This is to avoid installing the Application Manager which can turn into the Creative Cloud. Please understand, that I would only be interested in installing any available updates manually. I have a standard retail CS6 serial number, not CS6 Extended. |
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